畜牧兽医学报杂志 北大期刊 CSCD期刊 统计源期刊

Bone Morphogenetic Protein 15 as a Candidate Gene for Prolificacy of Jining Grey Goat

作者：FENG Tao ZHAO You-zhang CHU Ming-xing DI Ran ZHANG Ying-jie FANG Li 刊期：2009年第S1期

On the basis of the ovine bone morphogenetic protein 15(BMP15)gene,two pairs of primers(PI and P2)were designed to amplify exons 1 and 2 of the BMP15 gene in five randomly selected does of both Angora and Jining Grey goats.The sequences of BMP15 exon 1(P1 amplification)of Angora and Jining Grey goats were identical.T...

Preface

作者：J.Wen 刊期：2009年第S1期

<正>It has been 53 years since the first volume of Acta Veterinaria et Zootechnica Sinica(Chinese Journal of Animal and Veterinary Sciences,CJAVS)was published.The Journal has earned its reputation by presenting high-level academic and instructive papers,technical reports,and achievements in Chinese,but its inf...

Information for Contributors

刊期：2009年第S1期

<正>Acta Veterinaria et Zootechnica Sinica(Chinese Journal of Animal and Veterinary Sciences,CJAVS)is one of the earliest scholarly journals in animal and veterinary sciences of China(first published in 1956).The CJAVS(now published monthly),is supervised by the China Association for Science and Technology(CAST...

Comparison of Analyses of Genetic Structure among Chinese Indigenous Chicken Breeds using Distance-based and Model-based Methods

作者：LI Hui-fang CHEN Kuan-wei HAN Wei ZHANG Xue-yu GAO Yu-shi CHEN Guo-hong ZHU Yun-fen WANG Qiang 刊期：2009年第S1期

The Nei’s improved genetic distance(DA)and gene flow(Nm)were measured using sixteen microsatellite markers.Dendograms based on DA genetic distance using the neighbor-joining(NJ)method and STRUCTURE program were constructed to analyze the genetic structure and relationship among 10 Chinese indigenous chicken breeds.T...

Restriction Fragment Length Polymorphism in the MC4R Gene and Its Genetic Effects on Carcass and Feather-down Traits in Geese

作者：CHEN Hong-quan HUANG Hua-yun CHEN Hua ZHANG Tong-yan 刊期：2009年第S1期

Complete Sequence of Yak Mitochondrial D-loop Region and Its Taxonomic Status in Bovinae

作者：LI Qi-fa LI Yin-xia DONG Li-yan ZHAO Xing-bo PAN Zeng-xiang LI Ning XIE Zhuang 刊期：2009年第S1期

Primers based upon the mitochondrial genome sequences of Bos taurus were used to amplify and sequence the complete mitochondrial D-loop region of Jiulong yak(Poephagus grunniens).The results showed that the length of D-loop was 893 bp,with 87.4%homology to the Bos taurus D-loop sequence;there were 17 bp deletion.Usin...

Effect of Xylanase on mRNA Expression of Cationic Amino Acid Transporters in Intestines of Broilers

作者：FENG Ding-yuan TAN Hui-Ze WANG Xiu-qi ZOU Shi-geng ZUO Jian-jun HUANG Yan-hua DONG Ze-min ZHANG Chang-ming YE Hui 刊期：2009年第S1期

480 healthy 1-day-old male yellow-feathered chickens were selected and assigned randomly into groups A and B,each having 6 pens with 40 birds per pen.The birds in group A were fed with wheatbased diet and group B with wheat-based diet supplemented with xylanase(1.2×l0~4 U/kg diet).On day 16,two birds per replication ...

The Effect of Parity on Conjugated Linoleic Acid(CLA) Content in Milk Fat and CLA-Desaturase Index from Holstein Dairy Cows

作者：HOU Jun-cai LIU Fei LIU Yan-ping HUO Gui-cheng REN Da-xi TI Wei-gang 刊期：2009年第S1期

The performance of lactating dairy cows and composition of the fatty acids in milk fat were measured to investigate the effect of parity on conjugated linoleic acid(CLA)in milk fat and△~9-desaturase indices from Holstein dairy cows.Milk samples of two hundred and thirty-five lactating Holstein dairy cows in north Ch...

ArcA Gene Expression Level of Actinobacillus pleuropneumoniae in Anaerobic Culture

作者：DUAN Li-li WEN Xin-tian CAO San-jie HUANG Xiao-bo 刊期：2009年第S1期

In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal standard.The expression of ArcA was u...

Expression of Major B-cell Epitopes within the 2C Non-structural Protein of FMDV and Their Immunoreactivity

作者：FU Yuan-fang LU Zeng-jun TIAN Mei-na ZHANG Xiao-li LIU Zai-xin CAI Xue-peng 刊期：2009年第S1期

A pair of specific primers was designed from the 2C gene sequence of foot-and-mouth disease virus(FMDV)for amplification of a fragment including 174bp of the 5’-end and 279bp of the 3’-end of the2C gene,which encoded an abundance of known B-cell epitopes of the protein.The amplified fragment was inserted into pET-3...

Infectivity Analysis of Cloned Genomic DNA of P1 Agent in vitro

作者：WEN Li-bin HE Kong-wang YANG Han-chun 刊期：2009年第S1期

The ultrastructure of porcine kidney(PK)-15 cells was examined after lipofectamine-aided transfection of the molecular clone of the P1 agent.PK-15 cells transfected with the tandem dimer of the P1molecular DNA clone had numbers of intracytoplasmic inclusions,and a few cells had intranuclear inclusions.Intracytoplasmi...

The Effects of Insulin and Prolactin on an Epithelial Cell Line from Mammary Gland of Dairy Goat

作者：TONG Hui-li GAO Xue-jun LI Qing-zhang YAN Yun-qin 刊期：2009年第S1期

Mammary epithelial cells with lactational function can be a valuable cellular model for research of the development and regulation of the mammary gland.This paper describes some aspects of function of an epithelial cell line from the mammary gland of the dairy goat.SDS-PAGE,triglyceride and lactose content of culture...

Effects of Bcl-2 Gene Interference on the Apoptosis,Proliferation and Progesterone Secretion of Goose Follicular Granulosa Cells

作者：CHEN Xiu-ping CHEN Feng-jian JIANG Xun-ping LI Zhao-guo DING Jia-tong 刊期：2009年第S1期

Establishing an ELISA,Based on the HA1 Protein,for Detecting Antibodies against H3N2 Subtype Swine Influenza Virus

作者：DING Xuan-ya QIAO Chuan-ling CHEN Yan YANG Huan-liang XIN Xiao-guang HAN Qing-gong CHEN Hua-lan 刊期：2009年第S1期

The HA1 gene of H3N2 subtype swine influenza virus(SIV)was cloned into the expression plasmid pET-30a,the recombinant plasmid was named pET-HAl.This was transformed into E.coli BL21(DE3),and expressed by induction with IPTG.The expressed HA protein was identified by SDS-PAGE and Western blotting which showed the prot...

The Influence of SelW on GSH and GPx after Post-Transcriptional Gene Silencing in Mouse Skeletal Muscle Cells

作者：WANG Xiao-long XU Kai QIN Ou-ju CHEN Xiao-feng YANG Chuan-ping 刊期：2009年第S1期

The expression of Selenoprotein W(SelW)in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione(GSH)and the activity of glutathione peroxidase(GPx).SelW knock-down was performed by RNA interference(RNAi)in cultured muscle cells and verified by Real-time PCR and W...