中国药理学与毒理学杂志 北大期刊 统计源期刊

IcarisideⅡ alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 celloxidative injury by activating Nrf2 / SIRT3signaling pathway

作者：FENG; Lin-ying; GAO; Jian-mei; LIU; Yuan-gui; SHI; Jing-shan; GONG; Qi-hai 刊期：2018年第09期

超极化激活环核苷酸门控阳离子通道功能抑制剂ZD7288通过凋亡途径对大鼠脑缺血再灌注损伤发挥神经保护作用

作者：张文娟; 曾晓莉; 赵博; 陈静; 李自成; 何治 刊期：2018年第09期

目的运用大脑中动脉线栓(MCAO)法建立大鼠缺血2h/再灌注22h的脑缺血再灌注损伤模型,从凋亡途径探讨超极化激活环核苷酸门控阳离子(HCN)通道功能抑制剂ZD7288对大鼠脑缺血再灌注损伤的神经保护作用。方法①健康成年SD雄性大鼠,随机分为4组:假手术组、模型组、ZD7288组和MK-801组,通过MCAO法制备右侧大脑中动脉缺血2h/再灌注22h脑缺血模型。于缺血...

Ginsenoside Rg1 protects against is chemiareperfusion-induced neurotoxicity throughmiR-144/Nrf2/ARE pathway

作者：CHU; Shi-feng; ZHANG; Zhao; ZHOU; Xin; HE; Wen-bin; CHEN; Nai-hong 刊期：2018年第09期

OBJECTIVE Ginsenoside Rg1 (Rg1), a purified compound from Panax ginseng, has been well documented to be effective against ischemia/reperfusion (I/R) neurotoxicity. However, the underlying mechanism is still obscure. METHODS The anti-I/R effect of Rg1 were investigated in vitro and in vivo, and the dynamics of nuclear...

探讨TRPP2和基质相互作用因子1在高盐诱导高血压大鼠脑血管平滑肌细胞中的变化及对血管收缩的调节作用

作者：姜婉; 叶立; 杨欣玥; 沈兵; 汪凯 刊期：2018年第09期

目的研究高盐诱导高血压大鼠基底动脉血管平滑肌细胞收缩功能的改变,探讨钙库操纵的钙内流(SOCE)及其组成分子TRPP2和基质相互作用因子1(STIM1)对血管收缩的调节作用。方法应用鼠尾血压测量仪检测不同时间大鼠血压的变化;应用离体血管培养技术,分别特异性地敲低基底动脉血管中TRPP2和STIM1的蛋白表达;应用离体血管张力检测实验,利用内皮素1(ET-1...

Apelin-17对脑缺血性脑卒中后侧支循环的作用

作者：姜婉; 胡伟; 叶立; 田仰华; 赵韧; 杜鹃; 沈兵; 汪凯 刊期：2018年第09期

目的通过研究血浆中apelin-17水平和侧支循环之间关联的影响,并使用血管张力检测apelin-17对脑动脉收缩和动物模型中其对缺血性脑卒中的作用,探讨apelin-17在脑缺血再灌注损伤中的作用及相关机制。方法在临床上收取60名缺血性脑卒中患者伴良好侧支循环(A组)、8名缺血性脑卒中患者伴差侧支循环(B组)及22名健康对照(C组)的血标本,通过酶联免疫吸附...

微RNA-1诱导心肌梗死小鼠海马神经元微管损伤的分子机制

作者：孙琳琳; 段明靖; 马继超; 艾静 刊期：2018年第09期

目的观察心肌梗死(MI)小鼠心脏高表达的微RNA-1(miR-1)能否进入脑组织,影响神经元功能及其分子机制。方法采用左冠状动脉结扎(LCA)4周建立C57BL/6小鼠MI动物模型。透射电子显微镜技术观察海马微管的超微结构。Western蛋白印迹实验和PCR实验检测miR-1水平和TPPP/P25蛋白表达。双荧光素酶报告实验技术、AMO-1和miR-masking离体转染技术和海马立体...

Icariside Ⅱ, a PDE5 inhibitor, attenuates cerebral ischemia/reperfusion injury through activating BDNF/TrkB/CREB signaling pathway

作者：XU; Fan; LYU; Chun; DENG; Yan; LIU; Yuan-gui; GONG; Qi-hai; SHI; Jing-shan; GAO; Jian-mei 刊期：2018年第09期

OBJECTIVE To explore the effects and mechanism of icariside Ⅱ(ICS Ⅱ),a pharmacologically active compound derived from herbal Epimedii with previous study-proved phosphodiesterase 5(PDE5) inhibitors,was investigated in vivo using a middle cerebral artery occlusion/reperfusion(MCAO/R) model in rats and in vitro using...

Chemokines play complex roles in cerebral ischemia

作者：CHEN; Chen; CHU; Shi-feng; LIU; Dan-dan; ZHANG; Zhao; KONG; Ling-lei; ZHOU; Xin; CHEN; Nai-hong 刊期：2018年第09期

Ischemic stroke (IS) is a disease caused by deficiency of blood and oxygen in focal or complete brain, followed by inflammation cascade and other pathological reactions, which finally lead to irreversible damage to the cerebrum. For the inflammation is a key progress at the initiation of ischemia and poststroke, and ...

CKLF1 aggravates focal cerebral ischemia injury at early stage partly by modulating microglia/macrophage toward M1 polarization

作者：CHEN; Chen; CHU; Shi-feng; AI; Qi-di; ZHANG; Zhao; GUAN; Fei-fei; WANG; Sha-sha; DONG; Yi-xiao; ZHU; Jie; JIAN; Wen-xuan; CHEN; Nai-hong 刊期：2018年第09期

OBJECTIVE To investigate the CKLF1 mediated expression of microglia/macrophage phenotypes in vitro and in vivo, discussing the involved pathway. METHODS In vitro, primary microglia isolated from mice cortex were used to study the effects of CKLF1 by qPCR analysis and immunofluorescence staining. In vivo, WT C57 and C...

损伤脑内神经血管网络重构的细胞和分子机制的研究

作者：潘之光; 邱梅红; 陈献华; 孙凤艳 刊期：2018年第09期

缺血性脑卒中发生后脑内的病理十分复杂,包括急性和慢性神经细胞的死亡和再生修复。已知神经血管网络重构在损伤脑修复中起关键作用。本课题组的系列研究发现,缺血损伤脑区损伤的细胞除了发生不可逆的死亡外,还存在神经元和血管的新生及星形胶质细胞增殖。有趣的是,这些血管和星形胶质细胞在神经元新生中发挥重要的调节作用。缺血损伤脑内活化的...

Oleoylethanolamide protects against isch-emic stroke by modulating microglia M1/M2 polarization in PPARα-dependent manner

作者：XU; Lan-xi; ZHANG; Hui-jun; ZHOU; Hao; ZHUO; Ren-gong; ZHANG; Yi-jiao; JIANG; Xun; JIN; Xin; YANG; Li-chao 刊期：2018年第09期

OBJECTIVE Oleoylethanolamide (OEA) has shown neuroprotective effect in treating acute and chronic ischemic stroke. However, it is unclear whether OEA is able to modulate microglia / macrophage polarization, which has recently been documented to be important in the pathology of ischemic stroke. This study explored the...

IMM-H004 therapy for permanent focal isch-emic cerebral injury and cardiopulmonarycomplications via CKLF1 / CCR4-mediatedinflammation pathway

作者：AI; Qi-di; CHEN; Chen; CHU; Shi-feng; ZHANG; Zhao; LUO; Yun; GUAN; Fei-fei; ZHANG; Shuai; WANG; Sha-sha; YAN; Xu; CHEN; Nai-hong 刊期：2018年第09期

OBJECTIVE To investigate the effects of IMM-H004 on permanent focal cere-bral ischemia injury and associated cardiopulmonary complications, further elucidating the molecular mechanisms. METHODS The effects of IMM-H004 were investigated in wild-type (WT)and CKLF1^(-/-)rats. The effects of IMM-H004 on ischemic stroke i...

缺血神经元轴突线粒体逆向转运回胞体后被自噬清除

作者：郑艳榕; 张翔南; 陈忠 刊期：2018年第09期

目的研究缺血神经元轴突上的线粒体是否及如何被自噬清除。方法小鼠原代皮质神经元给予氧糖剥夺-复灌(OGD-Rep)处理,利用免疫荧光法观察轴突上线粒体数量的变化;利用微流体培养装置分离神经元轴突并对轴突线粒体进行特异性标记;利用活细胞共聚焦显微成像观察轴突线粒体的转运;最后利用电穿孔质粒转染法,在原代神经元中调控线粒体运动,观察其对线...

Circular RNA TLK1 aggravates neuronal injury and long-term neurological deficits via miR-335-3p/TIPARP after ischemic stroke

作者：WU; Fang-fang; YAO; Hong-hong 刊期：2018年第09期

OBJECTIVE Circular RNAs (circRNAs) are highly expressed in the brain and involved in various central nervous system diseases. However, the potential role of circRNAs in ischemic brain injury remains largely unknown. The present study investigated the functional significance of a circular RNA TLK1 (circTLK1) in cerebr...

缺血性脑白质损伤的治疗新靶点和新策略研究

作者：胡薇薇; 周怡亭; 张菁; 马婧; 陈忠 刊期：2018年第09期

大脑长期慢性缺血将可能导致脑白质损伤及认知功能损害,造成皮质下缺血性血管性痴呆。目前临床上缺乏针对性的有效药物,因此急需针对其发病机制寻找促进髓鞘再生、脑白质修复的治疗新靶点。本课题组研究发现,慢性缺血后脑室下区增殖的少突胶质前体细胞(OPC)向白质区迁移过程受到阻碍,导致了髓鞘再生过程受到抑制。进一步通过IL^-1β的受体IL^-1R1...