《生物技术》杂志2018年第06期期刊目录-发表之家

Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System Effector Protein HY 322 Gene of Vibrio alginolyticus

作者：Shanshan; LIANG; Mingjie; FAN; Weijian; LIANG; Huanying; PANG; Chuanhao; PAN; Dawei; SONG; Mingsheng; QIU; Jichang; JIAN 刊期：2018年第06期

In this study,Hy 322 gene was cloned from Vibrio alginolyticus.The total length of its gene was 969 bp,and it could encode 322 amino acids.The physicochemical properties,protein structure,genetic evolutionary relationship and antigenic characteristics of the effector protein Hy322 of V.alginolyticus HY9901 typeⅢsecr...
Advances in Plant Resistance to Viral Diseases Induced by Exogenous Double-stranded RNA

作者：Qiang; XIE; Junfeng; XIN; Longfei; YU; Xiao; LEI; Yonghui; ZHANG 刊期：2018年第06期

Application of exogenous double-stranded RNA(dsRNA)for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors.However,the instability of dsRNA is a major challenge as spraying naked dsRNA onto plants provides protection against hom...
Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea

作者：Huiqing; LIU; Yuantao; HUANG; Mengyu; DAI; Yimin; QIU; Gengfeng; ZHAO; Guoru; XIONG 刊期：2018年第06期

Sugarcane smut greatly influences yield and quality of sugarcane.HAS 1 gene in biocontrol strain HAS antagonistic to Sporisorium scitanminea was selected for prokaryotic expression vector construction and inducible expression,and the antagonistic effect of HAS1 protein was detected in vitro.The results showed that th...
Development of a Kind of Immunomagnetic Bead Kit to Separate and Enrich Salbutamol

作者：Yuping; WAN; Xiaosheng; WU; Fangfang; JIA; Jing; LI; Zhaoqin; WANG; Yuejun; FENG; Meihong; DU; Fangyang; HE 刊期：2018年第06期

An immunomagnetic bead kit to separate and enrich salbutamol was prepared through the reaction between salbutamol and 4-aminobenzoic acid.The kit had a catch of 20 ng/ml to salbutamol in samples,and showed no cross reaction with competitive drugs with structure and function similar to salbutamol:clenbuterol,ractopami...
Establishment of a Real-time Fluorescent Quantitative RTPCR Method for Detecting NP Gene of Class Ⅰ Newcastle Disease Virus(NDV)

作者：Junping; CAO; Xiaoquan; WANG; Han; CHENG; Xiaowen; LIU; Xiufan; LIU 刊期：2018年第06期

Newcastle disease( ND) is one of the most serious infectious diseases that infect the poultry industry.There is only one serotype of Newcastle disease virus( NDV),but NDVs can be divided into two distinct classes( class Ⅰ,and class Ⅱ) according to their genetic relationship.To develop a method for rapid quantitativ...
Analysis of HMW-GS in Pedigree of Longdong Dryland Winter Wheat Varieties

作者：Xiao; YANG; Zicheng; LIU; Guanghong; JIA 刊期：2018年第06期

The composition of high molecular weight glutenin subunits(HMW-GS)was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryland winter wheat germplasm resources had N,7+8,2+12 as the dominant HMW-GS composition(42.86%),while th...
Effect of Exogenous Spermidine Concentration on Chilling Tolerance in Cucumber

作者：Hong; WANG; Yuping; JIANG; Ying; WANG; Weimin; ZHU 刊期：2018年第06期

Chilling stress was one of the most sever abiotic stress to restrict cucumber plant growth.The effects of different concentrations of spermidine(0.1,0.5,1 and 5 mM)on photosynthetic characteristics,antioxidant(catalase,ascorbate peroxidase,superoxide dismutase and peroxidase)enzyme activities,malondialdehyde(MDA)cont...
Screening of Tomato Resources Resistant to Tomato Gray Mold

作者：Qiang; ZHANG; Wenting; DAI; Hongbin; WU; Hui; YANG; Xinwen; JIN 刊期：2018年第06期

This study was conducted to screen tomato resources resistant to gray mold for the first time by in-vitro stem inoculation method.The results showed that Solanum habrochaites T2-07-316 was highly resistant to gray mold,and had the relative stem infection rate and relative stem rot expansion rate of 0 on the 6 th day ...
High-yield Production Techniques of High-quality and Early Maturity Hybrid Rice Xiangzaoyou 2017

作者：Guozhong; WANG; Naiqiao; HONG; Guofu; ZHOU; Qianyi; XIAO; Enrui; CHEN; Yi; ZHOU 刊期：2018年第06期

Xiangzaoyou 2017 is an excellent early maturing variety bred from the combination of Neixiang 3A and the self-fertile restorer QN 2017 by Qiannan Institute of Agricultural Sciences of Guizhou Province,and it has been approved by the Guizhou Crop Variety Approval Committee(Approval No.:Qianshendao 2006001).In order to...
Role and Mechanism of Action of Exogenous Salicylic Acid and Sodium Molybdate in Improving Cold Tolerance of Bougainvillea glabra

作者：Yongfu; ZHANG; Yanfen; NIU; Shengjing; PENG 刊期：2018年第06期

This study was conducted to comprehensively evaluate the effects of salicylic acid and sodium molybdate on cold tolerance of an ornamental plant Bougainvillea glabra and to provide a theoretical guidance for landscape maintenance.B.glabra plants were treated with 0.5 mmol/L salicylic acid and 2.0μmol/L alone or in co...
Stalk Rot Resistance Analysis and Yield Loss Evaluation on 25 Maize Varieties and Utilization of Resistant Germplasm Jun M9

作者：Ruiqian; LU; Hailei; JIN; Shoulin; ZHANG; Jinkui; ZHANG; Changjian; LI; Zhifang; ZHANG 刊期：2018年第06期

Stalk rot outbroke in Henan Province in 2014 on maize,and the disease conditions of 25 varieties were investigated.According to national maize variety disease resistance identification standards,Jundan 509 was a resistant variety;Yudan 606,Jinsai 38 and Jundan 3136 were moderately-resistant varieties;and other 21 var...
Impact of Fungicide Treatments on the Constitution of Unsound Wheat Kernels

作者：Ze; LIU; Yiliang; QIAN; Li; ZHAO; Xianfang; HE; Jianlai; WANG 刊期：2018年第06期

Nine fungicides were applied,either through seed-dressing or post-anthesis foliar-spray,to eight winter wheat varieties to understand their influences on the constitution of unsound kernels during the 2017-2018 growth season in Funan Experimental Station,Anhui,P.R.China.Three unsound kernel items,i.e.Fusarium-damaged...
Seedling Raising of Moringa oleifera in Chongqing

作者：Zhengchun; WANG; Haiyan; JIANG; Jing; XIN; Wanlian; XIANG 刊期：2018年第06期

The tests of raising seedlings of Moringa oleifera were carried out in autumn and spring in Chongqing.The results showed that in the case of container seedling raising,the seedling emergence rate in autumn was 90%.Due to the large humidity and extreme weather,and the limited management conditions such as ventilation,...
Research Progress and Prospect on Prevention and Control Measures of Sweet Potato Virus Disease

作者：Fang; DONG; Daowei; ZHANG; Chaofan; ZHANG 刊期：2018年第06期

Sweet potato virus disease is a serious biological threat to sweet potato,which seriously affects the development of sweet potato industry in China.This paper gives a brief introduction to the main defensive measures of sweet potato virus disease,such as cutting of virus infection source,killing of viral transmission...
Comparative Test on New Small Watermelon Varieties with Red Flesh under Soilless Culture Conditions

作者：Xuesong; YOU; Jianbo; ZENG; Li; ZHU; Chao; MA; Yanli; CHEN; Yunfei; LI; Ting; LI; Ying; ZHANG 刊期：2018年第06期

In order to screen out the small watermelon varieties with red flesh suitable for soilless cultivation and to meet people s requirements of high-quality watermelons,comparative tests were conducted on 6 small watermelon varieties with red flesh under soilless culture conditions in 2018.Based on the data of field trai...

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生物技术杂志统计源期刊

Biotechnology

Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System Effector Protein HY 322 Gene of Vibrio alginolyticus

Advances in Plant Resistance to Viral Diseases Induced by Exogenous Double-stranded RNA

Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea

Development of a Kind of Immunomagnetic Bead Kit to Separate and Enrich Salbutamol

Establishment of a Real-time Fluorescent Quantitative RTPCR Method for Detecting NP Gene of Class Ⅰ Newcastle Disease Virus(NDV)

Analysis of HMW-GS in Pedigree of Longdong Dryland Winter Wheat Varieties

Effect of Exogenous Spermidine Concentration on Chilling Tolerance in Cucumber

Screening of Tomato Resources Resistant to Tomato Gray Mold

High-yield Production Techniques of High-quality and Early Maturity Hybrid Rice Xiangzaoyou 2017

Role and Mechanism of Action of Exogenous Salicylic Acid and Sodium Molybdate in Improving Cold Tolerance of Bougainvillea glabra

Stalk Rot Resistance Analysis and Yield Loss Evaluation on 25 Maize Varieties and Utilization of Resistant Germplasm Jun M9

Impact of Fungicide Treatments on the Constitution of Unsound Wheat Kernels

Seedling Raising of Moringa oleifera in Chongqing

Research Progress and Prospect on Prevention and Control Measures of Sweet Potato Virus Disease

Comparative Test on New Small Watermelon Varieties with Red Flesh under Soilless Culture Conditions

建筑工程技术与设计

中学生物教学

电气技术

农业与技术

现代生物医学进展

电力电子技术

职业技术教育

中国职业技术教育

科学技术创新

山东工业技术

电脑知识与技术

电工技术

生物技术杂志 统计源期刊

Biotechnology

Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System Effector Protein HY 322 Gene of Vibrio alginolyticus

Advances in Plant Resistance to Viral Diseases Induced by Exogenous Double-stranded RNA

Identification of In-intro Antagonistic Activity of HAS1 Protein against Sporisorium scitanminea

Development of a Kind of Immunomagnetic Bead Kit to Separate and Enrich Salbutamol

Establishment of a Real-time Fluorescent Quantitative RTPCR Method for Detecting NP Gene of Class Ⅰ Newcastle Disease Virus(NDV)

Analysis of HMW-GS in Pedigree of Longdong Dryland Winter Wheat Varieties

Effect of Exogenous Spermidine Concentration on Chilling Tolerance in Cucumber

Screening of Tomato Resources Resistant to Tomato Gray Mold

High-yield Production Techniques of High-quality and Early Maturity Hybrid Rice Xiangzaoyou 2017

Role and Mechanism of Action of Exogenous Salicylic Acid and Sodium Molybdate in Improving Cold Tolerance of Bougainvillea glabra

Stalk Rot Resistance Analysis and Yield Loss Evaluation on 25 Maize Varieties and Utilization of Resistant Germplasm Jun M9

Impact of Fungicide Treatments on the Constitution of Unsound Wheat Kernels

Seedling Raising of Moringa oleifera in Chongqing

Research Progress and Prospect on Prevention and Control Measures of Sweet Potato Virus Disease

Comparative Test on New Small Watermelon Varieties with Red Flesh under Soilless Culture Conditions

建筑工程技术与设计

中学生物教学

电气技术

农业与技术

现代生物医学进展

电力电子技术

职业技术教育

中国职业技术教育

科学技术创新

山东工业技术

电脑知识与技术

电工技术

生物技术杂志统计源期刊