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作者:JINNOKiyokatsu; SAITOYoshihiro 刊期:2004年第04期
A typical analytical separation procedure has several important steps: sample preparation, isolation, identification, quantitation, statistical evaluation and final decision. Each step is always critical to obtain correct results to fulfill the analytical purpose. In these various steps sample preparation step is req...
作者:张玉奎 刊期:2004年第04期
The separation and identification of complex samples is an urgent task that analytical chemistry has to face. Chromatography, as an important analysis technique, has been widely applied to many fields, such as life science, environment, medicine, food and petrochemical engineering. Recently, with the progress of scie...
作者:HAGINAKAJun; SANBEHaruyo 刊期:2004年第04期
Molecular recognition plays an important role in biological and chemical processes. Since molecular imprinting techniques can afford complementary binding sites for a target molecule, the molecularly imprinted polymer (MIP) for the target molecule has been used for its specific recognition as chromatographic media, s...
作者:郑志侠; 林金明; 许华杰 刊期:2004年第04期
The separation of enantiomers is of great importance in biology, pharmaceutics, agriculture and environment. The different separation modes (i.e. capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary electrochromatography (CEC), etc) and many chiral selectors available make ca...
作者:HASHI; Yuki; 吕冬; 曹磊 刊期:2004年第04期
The flow diagram of this on-line sample pretreatment system is shown in Fig.1.
作者:王畅; 谢四刚; 杨军; 杨青; 许国旺 刊期:2004年第04期
As an important constituent in the biomembranes, phospholipids (PL) are a complex mixture of molecular species containing a variety of fatty acyl and head group compositions. In addition to their structural role, some phospholipids also participate in biological processes in various ways, such as cellular signaling[1...
作者:BABAYoshinobu 刊期:2004年第04期
1 Introduction Since human genome sequencing has been almost completed, human genome project will quickly move on to the post genome sequencing era, including single nucleotide polymorphism (SNP) analysis, functional genomics, mutation analysis, transcriptome analysis, proteome analysis, and metabolome analysis[1,2]....
作者:刘红霞; 宋鹃梅; 张书胜; MACKA; Miroslav; HADDAD; Paul; R 刊期:2004年第04期
Capillary electrophoresis (CE) has rapidly gained great interests among researchers in many different fields. One of these areas is the separation of small ions such as inorganic cations[1-9], anions[10-16], and low Mr organic molecules[17-20]. However, as the separation of ions by CE is depended on the difference in...
作者:王霞; 李文智; 柳春辉; 陈立仁; 李永民 刊期:2004年第04期
In recent years, the synthesis of chiral tetrahedral clusters has been studied extensively and various types are accessible[1,2], which are a kind of organometallic compounds with greatly growing interest due to their potential application to asymmetric reaction catalysts[3,4]. As an efficient separation technique fo...
作者:李薇; 车宝泉; 贡素萱; 陈政; 文大为; 廖一平; 刘虎威 刊期:2004年第04期
Aristolochic acid (AA) composed of AA-Ⅰ and AA-Ⅱ (Fig.1) is the major toxic component extracted from Aristolochia plants[1]. Recently, it has been proved by several researches that high doses of aristolochic acids (AA) can result in a severe kidney disease named Chinese herbs nephropathy (CHN)[2]. It is characteriz...
作者:KITAMORITakehiko 刊期:2004年第04期
1 Micro chemical system on chip Micro integrated chemical systems are expected as promising ultra high throughput chemical and bio processors with extremely small volume. Our research groups have proposed and developed the original methodologies for micro integration of general chemical systems as well the electropho...
作者:黄晓冬; 陈学国; 欧俊杰; 唐守万; 孔亮; 邹汉法 刊期:2004年第04期
Since their introduction in 1992 by Fréchet and Svec[1], monolithic supports as stationary phases in high performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have gained significant interest due to a number of unique properties. Their ease of preparation, high reproducibility, versatile...
作者:MANONariyasu; GOTOTakaaki; GOTOJunichi 刊期:2004年第04期
Bile acids are synthesized in the liver from cholesterol by the action of hepatic enzymes and excreted into the small intestine via the bile duct. In the intestinal lumen, they assist lipolysis and the absorption of fats by forming mixed micelles, and then return to the liver upon absorption in the ileum and proximal...
作者:李发美; 郭兴杰; 乔明曦; 熊志立; 周大炜 刊期:2004年第04期
Drugs in the body are bound to metabolizing enzymes, targets/receptors and transport proteins in certain extent. The binding of drugs to targets or receptors is mainly specific and responsible for its pharmacological and therapeutic effects. The metabolizing of drugs by enzyme involves both specific and non-specific ...
作者:关亚风; 王涵文; 刘文民 刊期:2004年第04期
Since the development of solid phase micro-extraction (SPME) method[1], many modifications have been made to improve the availability and application of the technique[2-5]. The in-tube SPME (ITSPME) was emerged and employed in high performance liquid chromatography (HPLC) as an efficient and simple preparation method...