摘要:目的:利用pEGFP质粒载体构建介导结缔组织生长因子(CTGF)短发夹RNA(short hairpin RNA,shRNA)表达的质粒.方法:分别设计3对有小发夹结构的两条DNA序列,经退火成互补双链,再克隆至带有U6启动子的质粒载体pEGFP中,构建重组体,转化DH5α菌株,提取质粒行酶切鉴定后,进行测序分析.结果:CTGF的sRN段被成功克隆到pEGFP质粒载体中,3个重组质粒shRNA编码序列与设计的片段完全一致,经酶切与测序证实构建成功.结论:成功构建了能表达CTGF shRNA的重组体,为下一步探索肝纤维化基因治疗的新途径打好基础.
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