摘要:从猪肺泡巨噬细胞(PAM)中提取总RNA为模板,采用RT-PCR法获得猪繁殖与呼吸综合征病毒(PRRSV)的受体CD163全基因,将该基因克隆到真核表达载体pcDNA3.1/V5-HIS A上,构建出真核重组质粒pcDNA3.1-CD163,经酶切和DNA测序证明获得了CD163基因,其序列与GenBank报道序列比较,核苷酸同源性为99.37%。将测序正确的CD163基因在脂质体LipofectamineTM2000介导下转染PK-15细胞,通过间接免疫荧光(IFA)检测到了CD163在PK-15中的表达。将转染的细胞感染PRRSV后,经IFA检测转染CD163的细胞能够被PRRSV感染。
注:因版权方要求,不能公开全文,如需全文,请咨询杂志社
热门期刊服务
China Petroleum Processing Petrochemical Technology Petroleum Science Chinese Optics Letters Chinese Journal of Chemical Physics Applied Mathematics:A Journal of Chinese Universities Chinese Geographical Science Acta Pharmacologica Sinica Research in Astronomy and Astrophysics Journal of Geographical Sciences Journal of Integrative Plant Biology Journal of Systems Science and Complexity Progress in Natural Science:Materials International