摘要:采用修改后的CTAB法获得了高质量的基因组DNA.利用RAPD标记技术对山葡萄7份种质进行鉴定,用4个引物(从30个引物中筛选)对试材进行PCR扩增,共扩增出30条谱带,平均每条引物产生7.5条谱带,其中21条谱带为多态性谱带,占总谱带数的70%.不同引物扩增的谱带数不同,范围在6~9条之间.利用4个引物扩增出的多态性谱带可以将7份山葡萄种质区分.
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