摘要:目的:利用siRNA干扰技术沉默SOST基因,观察其对大鼠成骨细胞的影响,探讨SOST,siRNA治疗骨质疏松症( OP )的可能性。方法:实验分对照组、空载体组、SOST-siRNA1组及SOST-siRNA2组,将成功构建的10μg SOST-siRNA1和SOST-siRNA2表达载体分别采用脂质体法转染大鼠成骨细胞,空载体组大鼠成骨细胞转染等剂量的pGFP-VRS空载体。转染后24 h、48 h、72 h和96 h荧光倒置显微镜下观察细胞转染效率,实时荧光定量RT-PCR法检测4组大鼠成骨细胞中SOST-mRNA的表达水平,ELISA法检测4组细胞上清中碱性磷酸酶、Ⅰ型胶原蛋白表达水平变化。结果:成功构建SOST-siRNA 质粒载体, SOST-siRNA 可以抑制大鼠成骨细胞中SOST-mRNA的表达;促进细胞上清中Ⅰ型胶原蛋白表达。结论:SOST-siRNA抑制大鼠成骨细胞中SOST-mRNA的表达后,可以促进细胞上清中Ⅰ型胶原蛋白表达,同时促进成骨细胞的发育,提示其对OP有一定的治疗作用。
注:因版权方要求,不能公开全文,如需全文,请咨询杂志社
热门期刊服务
China Foundry Research in Astronomy and Astrophysics Neural Regeneration Research Journal of Semiconductors Frontiers of Information Technology Electronic Engineering Journal of Environmental Sciences Progress in Natural Science:Materials International China Petroleum Processing Petrochemical Technology Control Theory and Technology Journal of Harbin Institute of Technology Earthquake Engineering and Engineering Vibration Hepatobiliary Pancreatic Diseases International