摘要:目的观察c-myc癌基因在体外对胶质瘤细胞血管内皮生子因子(VEGF)表达的影响。方法构建以c—myc基因为靶向的shRNA干扰质粒pCMYC,同时构建pHK质粒作为阴性对照,采用逆转录.聚合酶链反应(RT—PCR)方法检测各组细胞中c-myc及VEGFmRNA水平的表达变化,采用免疫细胞化学法及Westernblot法分别检测3组细胞中c-myc及VEGF蛋白的表达。结果(1)IN500A—pCMYC细胞组c—myc与VEGFmRNA表达降低(0.273±0.028,0.443±0.048),与IN500A细胞组(1.185±0.145,1.116±0.072)及IN500A—pHK细胞组(1.098±0.128、1.208±0.133)比较差异均有统计学意义(P〈0.01)。(2)免疫细胞化学染色结果显示,IN500A-pCMYC细胞组c—myc与VEGF阳性细胞表达率均显著降低,为(24.52±4.39)%及(23.52±3.44)%,与IN500A及IN500A-pHK细胞组比较差异有统计学意义(P〈0.05)。(3)Westernblot法检测结果显示IN500A-pCMYC细胞中c-myc与VEGF的蛋白表达降低(0.221±0.041、0.2844-0.025),与IN500A细胞组(1.821±0.191、1.531±0.213)及IN500A-pHK细胞组(1.650±0.110、1.820±0.122)比较差异均有统计学意义(P〈0.01)。结论沉默c—mye基因能够抑制胶质瘤中VEGF的表达,从而降低肿瘤组织内的血管生成。
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