摘要:临床多采用ELISA双抗体夹心法,检测乙型肝炎表面抗原(HBsAg),其基本原理:包被抗原或抗体后,通过抗原抗体免疫学反应使酶标抗体结合到载体上,使结合的酶标抗体和游离的酶标抗体分离,洗去游离的酶标抗体,加人底物,生成有色产物,在一定温度下一定时间后终止反应,根据颜色深浅进行定性或定量分析.在ELISA方法中,应用辣根过氧化物酶(HRP)作为标记酶最普及,用此方法检测HBsAg时,常出现一些错误的结果即假阳性或假阴性,本文对产生假阳性或假阴性的错误结果进行分析如下.
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