摘要:以香蕉枯萎病菌菌株为试验材料,采用SDS-CTAB法和高盐沉淀法提纯香蕉枯萎病菌基因组DNA.结果表明:高盐沉淀法是适合于香蕉枯萎病菌基因组DNA提取的方法.该方法提取的DNA OD260/280值显示产物纯度较高;经琼脂糖凝胶电泳得到一条带型较宽且清晰的DNA谱带,DNA浓度较高,基本无DNA碎带;不用RNase处理,已无RNA的干扰,无需任何纯化处理即可用于PCR扩增和RAPD分析.同时对DNA提取过程中的细节问题进行了探讨与分析.
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