摘要:目的 探讨肿瘤坏死因子-α(TNF-α)在生殖衰老期小鼠卵巢组织中的表达情况,并探索TNF-α对卵巢颗粒细胞的作用及机制。方法 应用免疫组化方法检测TNF-α在生殖衰老期小鼠卵巢组织中的表达;体外培养卵巢颗粒细胞,加入外源性TNF-α因子,应用TUNEL和Western Blotting检测颗粒细胞凋亡及内在机制。结果 生殖衰老小鼠卵巢组织中TNF-α表达明显高于3周龄小鼠;TNF-α处理小鼠颗粒细胞凋亡呈度重于3周龄小鼠;TNF-α处理小鼠细胞cleaved-Caspase3、cleavedCaspase8表达增高,NF-κB磷酸化水平增高。结论 生殖衰老小鼠卵巢组织中TNF-α水平升高促进了颗粒细胞凋亡,该凋亡与NF-κB通路可能有一定关系。
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