摘要:目的探讨ELISA法和胶体金免疫层析法(GICA法)在乙肝表面抗原检测中的优缺点和应用价值。方法随机选取进行血清检测的600例血清标本,同时采用ELISA法和胶体金免疫层析法检测乙肝表面抗原,比较两种检测方法的灵敏度及差异性。结果 ELISA法检测阳性率为5.33%,灵敏度为96.97%,特异度为100%;胶体金免疫层析法检测阳性率为5.0%,灵敏度为90.90%,特异度为99.82%;两种方法检测阳性结果符合率为90.63%。结论胶体金免疫层析法灵敏度低,特异性基本一致,ELISA法检测的阳性率准确、灵敏度高为突出优点,是目前各级实验室应用最广泛的HBsAg检测方法。
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