摘要:目的探讨n-6/n-3多不饱和脂肪酸(PUFAs)对子宫内膜癌Ishikawa细胞增殖及细胞中哺乳动物雷帕霉素靶蛋白(mammMiantargetofrapamycin,mTOR)、血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)表达的影响。方法用不同比例的n-6/n-3PUFAs处理细胞,MTr法检测细胞增殖能力变化;RT—PCR检测细胞mTOR、VEGF的mRNA表达情况。结果单纯n-6PUFAs和10:1n-6/n-3PUFAs能够明显促进Ishikawa细胞增殖,mTOR、VEGF的mRNA表达增加(P〈0.05);单纯n-3PUFAs和1:1n-6/n-3PUFAs则显著抑制细胞增殖,同时使VEGF的mRNA表达下降(P〈0.05);mTOR与VEGFmRNA的表达呈正相关(r=0.94,P〈0.05)。结论n-6/n-3PUFAs可能通过影响mTOR信号调节VEGF表达,从而对Ishikawa细胞增殖产生影响。
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