摘要:目的:检测低分子柑橘果胶(low modified citrus pectin,Lmcp)对子宫内膜癌细胞株Ishikawa中凋亡调控因子Survivin蛋白表达的影响。方法:应用Western blot方法检测经不同浓度Lmcp(0、100、300、500nmol/L)作用不同时间(0、6、12、36 h)后,各组Ishikawa细胞中Survivin蛋白的表达情况。结果:提高Lmcp作用浓度及延长Lmcp作用时间,均可使Ishikawa细胞中Survivin蛋白的表达减弱。结论:Lmcp可下调子宫内膜癌细胞Ishikawa中存活蛋白Survivin的表达,且此效果有剂量(0-500 nmol/L)和时间(0-36 h)依赖性。
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