摘要：[Objectives]This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide and study the antioxidant activity of S.nudus polysaccharide.[Methods]S.nudus polysaccharide was extracted by ultrasonic microwave assisted method,during which univariate factors including solid-liquid ratio,time,temperature,ultrasonic power,microwave power were investigated,to select optimal extracting conditions,which were also verified by an response surface optimization experiment(an four-factor three-level test);and the antioxidant activity of S.nudus polysaccharide was studied by determining its DPPH radical scavenging capacity.[Results]Through univariate factor investigation and response surface optimization test,it was found that the response surface results were consistent with univariate results.The optimized conditions were as follows:solid-liquid ratio at 1∶15,ultrasonic temperature at 60℃,ultrasonic power of 360 W and microwave power of 200 W,and the predicted polysaccharide extracting amount was 4.35 mg/g.Under the optimized extracting conditions,the average extracting amount of S.nudus polysaccharide was 4.38 mg/g,which is close to the theoretical predicted value(4.35 mg/g).And the results of this study also showed that different concentrations of polysaccharide solutions have different scavenging effects on DPPH free radical.At the polysaccharide concentration of 0.16 mg/ml,the maximum value of DPPH radical scavenging rate was 7.22%,indicating that polysaccharide has certain antioxidant capacity.[Conclusions]This method is a new trial with satisfactory results.

注：因版权方要求，不能公开全文，如需全文，请咨询杂志社