摘要:目的:探讨m TORC1/2抑制剂PP242对人胶质瘤细胞U251凋亡的影响及其机制。方法:用不同浓度的PP242处理胶质瘤U251细胞不同时间后,CCK8法测定细胞存活率,采用流式细胞术检测细胞凋亡情况,Western blot检测凋亡通路相关蛋白的变化。结果:PP242呈时间和浓度依赖性地抑制人胶质瘤细胞的生长,促进细胞凋亡。同时PP242促进caspase依赖的凋亡,使Bax、cleaved caspase-3和cleaved caspase-9等蛋白表达上调,Bcl-2表达下调。结论:mTORC1/2抑制剂PP242通过调控凋亡蛋白的表达来诱导胶质瘤细胞的凋亡。
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