摘要:目的:探讨肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)对人脱落乳牙牙髓干细胞(stem cells from human exfoliated deciduous teeth,SHED)促破骨细胞形成能力的影响。方法:通过酶消化法体外分离、培养处于生理性根吸收时期的自然脱落乳牙牙髓干细胞;建立SHED与破骨前体细胞外周血单个核细胞(peripheral blood mononuclear cells, PBMCs)的间接共培养模型,在破骨诱导培养液中加入0、5、10、50、100 ng/m L不同浓度的TNF-α,通过实时定量RT-PCR和Western免疫印迹检测破骨相关基因及核转录因子κB(nuclear factor-κB,NF-κB)信号通路相关基因的表达。采用SPSS 19.0软件包对数据进行统计学分析。结果:在10 ng/mL的TNF-α刺激下,PBMCs中破骨相关蛋白CTSK和TRAP的表达水平均显著增高。Western免疫印迹和实时定量RT-PCR检测结果显示,SHED胞质内p-IκBα和胞核内p65蛋白、基因的表达水平在10 ng/m L的TNF-α刺激下显著高于无TNF-α刺激组。结论:炎症细胞因子TNF-α通过NF-κB信号通路对SHED促破骨细胞形成能力具有调控作用。
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