摘要:目的:研究Smad蛋白在牙本质涎磷蛋白(dentin sialophosphoprotein, DSPP)基因表达中的作用.方法:细胞培养,瞬时转染和报告基因检测.结果:转化生长因子β1(transforming growth factor-β1,TGF-β1)抑制DSPP基因启动子活性.过表达野生型Smad3蛋白显著增强了TGF-β1对DSPP基因表达的转录抑制,而过表达Smad3突变型蛋白则显著抑制了TGF-β1对DSPP基因启动子活性的影响.过表达Smad2野生型或突变型蛋白对TGF-β1抑制DSPP基因启动子活性无明显影响.结论:Smad3在介导TGF-b1对DSPP基因的转录调控中发挥重要的作用.
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