摘要:用禽脑脊髓炎灭活疫苗免疫28日龄SPF鸡10只,5只对照不免疫,免疫后21d采血分离血清,ELISA抗体效价检测,采血后马上进行攻毒,攻毒后观察统计发病情况,在试验鸡出现典型症状及攻毒后21d试验结束日,存活鸡全部采血测抗体,对比抗体与攻毒保护效果之间的关系.结果表明:攻毒前免疫鸡ELISA抗体阳性率为80%,攻毒保护效果〉80%,对ELISA抗体阳性鸡攻毒均有保护.
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