摘要:目的体外构建结缔组织生长因子(CTGF)序列特异性小干扰RNA表达载体,转染导入后研究其对人瘢痕疙瘩成纤维细胞(HKFs)胶原蛋白分泌的影响。方法体外构建CTGF序列特异性小干扰RNA质粒表达载体,Dosper脂质体转染人瘢痕疙瘩成纤维细胞后,观察加入10 ng/mL剂量的转化生长因子(TGF-β1)刺激前后胶原蛋白水平的变化。结果转染小干扰RNA质粒后,胶原蛋白分泌水平降为正常表达的51.6%;经TGF-β1刺激后,蛋白表达仍无明显变化。结论小干扰RNA质粒表达载体可抑制瘢痕疙瘩成纤维细胞胶原蛋白分泌,为临床上瘢痕疙瘩的基因治疗提供理论依据。
注:因版权方要求,不能公开全文,如需全文,请咨询杂志社
热门期刊服务
China Foundry Research in Astronomy and Astrophysics Neural Regeneration Research Journal of Semiconductors Frontiers of Information Technology Electronic Engineering Journal of Environmental Sciences Progress in Natural Science:Materials International China Petroleum Processing Petrochemical Technology Control Theory and Technology Journal of Harbin Institute of Technology Earthquake Engineering and Engineering Vibration Hepatobiliary Pancreatic Diseases International