摘要:利用PCR-SSCP及DNA测序技术,对632只河南县欧拉型藏羊、祁连县高原型藏羊和贵南县黑藏羊的MyoG基因exonⅠ多态性进行检测并分析多态位点与体尺性状的相关性。结果表明,MyoG基因exonⅠ109位存在A→C的碱基突变,存在CC、CA、AA3种基因型,该突变导致MyoG基因exonⅠ37号位谷氨酸(GAG)→丙氨酸(GCG)的错义突变;该位点在3个藏羊群体中均处于中度多态;最小二乘法分析表明,河南县欧拉羊群体中CC基因型个体的体重、体高和体长均显著高于AA基因型个体(P〈0.05),祁连县高原型藏羊群体中CC基因型个体的体重显著高于AA基因型个体(P〈0.05),贵南县黑藏羊群体中CC基因型个体的体长显著小于CA基因型和AA基因型个体(P〈0.05)。
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