摘要：通过"纸色谱-色斑洗脱比色法"定量测定发酵液中L-缬氨酸的实验研究,考察了测定波长、色斑洗脱时间、温度、洗脱液、稳定性等因素对测定效果的影响,确定了定量测定条件.此方法的测定结果与高速氨基酸分析仪测定结果相吻合,方法回收率为93.45%.
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