摘要:目的建立测定人血浆和尿液中美罗培南含量的RP-HPLC法。方法血浆样品经乙腈沉淀蛋白,二氯甲烷2次提取去杂质,取水相进样;尿液稀释后直接进样。以甲硝唑为内标,色谱柱为Capcell Pak MG C18(4.6 mm×150 mm,5μm)柱,流动相为乙腈-0.02 mol/L NaH2PO4溶液(体积比10∶90),流速为1.0 mL/min,检测波长为298 nm,柱温为30℃。结果美罗培南在血浆样品和尿样中的线性范围分别为0.2~100.0μg/mL和2~1 000μg/mL,提取回收率平均值分别为80.4%和101.1%,方法回收率平均值分别为99.2%和100.3%,日内、日间RSD值均低于8.8%。结论该方法简便、灵敏、专属性强,能满足人血浆和尿液中美罗培南含量测定及药动学研究的需要。
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