摘要:目的探讨STAT3对弓形虫排泄分泌抗原(ESA)抑制星形孢菌素诱导Raw264.7细胞凋亡的影响。方法利用星形孢菌素制作Raw264.7细胞凋亡模型,通过RNA干扰STAT3表达,弓形虫ESA处理细胞,蛋白质印记法检测STAT3、p STAT3、PARP、Cleaved Caspase-3和Cleaved Caspase-9蛋白表达水平。结果弓形虫ESA呈浓度依赖性降低Raw264.7细胞Caspase-3活性(P〈0.01),上调p STAT3表达;经STAT3 si RNA转染后,弓形虫ESA对p STAT3、Cleaved PARP、Cleaved Caspase-3和Cleaved Caspase-9表达无明显影响。结论 STAT3是弓形虫ESA抑制细胞凋亡的关键因素之一。
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